Human efflux transport of testosterone, epitestosterone and other androgen glucuronides
نویسندگان
چکیده
منابع مشابه
Stability studies of testosterone and epitestosterone glucuronides in urine.
The stability of testosterone glucuronide (TG), epitestosterone glucuronide (EG) and the T/E ratio in urine has been studied. Samples were analyzed by gas chromatography coupled to mass spectrometry (GC/MS). Urine samples were submitted to a solid-liquid cleanup followed by extraction of unconjugated testosterone (T) and epitestosterone (E) with tert-butyl methyl ether (free fraction). The rema...
متن کاملDevelopment and method validation for testosterone and epitestosterone in human urine samples by liquid chromatography applications.
An isocratic high-performance liquid chromatographic method for the determination of testosterone (T) and epitestosterone (ET) in human urine using liquid-liquid or solid-phase extraction (SPE) is developed and validated. The optimum separation is achieved using a Hypersil C(18) column, water-acetonitrile (57:43, v/v) as the mobile phase and UV-absorbance detection at 245 nm. The recoveries obt...
متن کاملCandida albicans in urine can produce testosterone: impact on the testosterone/epitestosterone sports drug test.
structure (4, 5). -NAGA deficiency is one of the rarest and probably the most heterogeneous of lysosomal storage disorders (3, 7). At present, only nine patients are known from six families. Of these, infants (four patients) and adults (five patients) display obviously different phenotypic expression. Adults commonly have angiokeratoma, loss of hearing, dizziness, and cutaneous sensory disturba...
متن کاملEpitestosterone in human blood and prostatic tissue.
Epitestosterone, a C19-steroid with anti-androgenic activity, was determined in the plasma of 234 boys and men from the ages of 6-86 years, and in the prostate tissue of 15 men 55-82 years of age. It was documented that, while in adulthood the concentration of epitestosterone is about ten times lower than the concentration of testosterone, in the pre-pubertal period the level of epitestosterone...
متن کاملDetermination of urinary testosterone and epitestosterone in men, women, and children.
A new, relatively simple method for simultaneous determinations of urinary testosterone and epitestosterone is described. The novel feature is the periodic oxidation of testosterone and epitestosterone glucuronides with sodium metaperiodate. The chromatographic purification of the ether extracts consisted of alumina-column chromatography, followed by thin-layer chromatography on silica gel of f...
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ژورنال
عنوان ژورنال: The Journal of Steroid Biochemistry and Molecular Biology
سال: 2020
ISSN: 0960-0760
DOI: 10.1016/j.jsbmb.2019.105518